منابع مشابه
New enzymatic colorimetric assay for total homocysteine.
References 1. Linder MW, Prough RA, Valdes R. Pharmacogenetics: a laboratory tool for optimizing therapeutic efficacy. Clin Chem 1997;43:254–66. 2. Raimundo S, Fischer J, Eichelbaum M, Griese EU, Schwab M, Zanger UM. Elucidation of the genetic basis of the common ‘intermediate metabolizer’ phenotype for drug oxidation by CYP2D6. Pharmacogenetics 2000;10:577– 81. 3. Sachse C, Brockmoller J, Baue...
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Patients with vascular disease have significantly higher concentrations of plasma total cysteine (tCYS) than do healthy individuals (1–7). The present method is a new, rapid, and sensitive enzymatic colorimetric assay for tCYS in plasma samples and is homogeneous in that it avoids separation methods. The tCYS assay uses only the recombinant enzymes methionine , -lyase (rMETase) and Sadenosylhom...
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Homocysteine exists in human plasma as various (mixed) disulfides. Most plasma homocysteine (about 70%) is protein bound, probably via a disulfide bond to albumin, whereas homocysteine-cysteine mixed disulfide is the predominating form in the free fraction. We here present a method for the determination of total homocysteine, which includes both fractions. Plasma was initially treated with sodi...
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min were as follows: At 70 MPa (10 000 lb./in.), no pressure effect was evident; ;10% of maximal binding was observed at 140 MPa, 20% at 210 MPa, 50% at 280 MPa, and 80% at 420 MPa. Preincubation of the anti-p24 antibody alone (without the p24 antigen) at 420 MPa for 10 min did not result in any discernible decrease in absorbance, indicating that pressures as great as 420 MPa did not affect the...
متن کاملEvaluation of an enzymatic method to measure total homocysteine in plasma.
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2000
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/46.10.1686